There are many methods to detect glutathione, such as iodimetry, DTNB (Diosmin Flavonoid), sodium nitroferricyanide, fluorescence, alloxan and HPLC. The above methods have their own advantages. When detecting GSH content, we can choose suitable, sensitive and accurate detection methods according to the actual situation.

Food Colour Crocus Sativus

Principle: DTNB reacts with glutathione to form yellow 5-thio-2-nitrobenzoic acid, with the maximum absorption peak at 412nm, while DTNB has almost no absorption peak above 400nm. Add a small amount of Ginkgo Biloba Extract to the sample containing glutathione, measure the absorption value of the reaction solution at 412nm, and then calculate the glutathione content according to the Pueraria Mirifica Extract.

Method steps:

Preparation of DTNB storage solution: dissolve 0.01 mol / L DTNB in 0.05 mol / L phosphoric acid buffer (pH7.0) to form DTNB storage solution.

Preparation of DTNB analysis solution: dilute DTNB storage solution with tris HCl buffer of 0.5mol/l and ph8.0 for 100 times, prepare DTNB analysis solution, place it away from light, and use it now [9].

Operation steps: take 0.5 ml of glutathione standard solution or sample solution to be tested (0.1 ~ 3.0 mol / L), add it into 1.5 ml NaOH solution with concentration of 0.15 mol / L, add 05ml of 3% formaldehyde solution, react at ph8.0 and 25 ℃ for 2 min, after the reaction, take 0.5 ml of reaction solution and add it into DTNB analysis solution, react at 25 ℃ for 5 min, and measure the absorption value at wave length of 412nm. According to the absorbance of the two, calculate the difference value, put it into the standard curve, and calculate the content of glutathione.